DNA Methylation Change of Oct-4 Gene Promoter Region during Bovine Preimplantation Early Embryos
2008
Ko, Y.G. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Kim, J.M. (Chungbuk National University, Cheongju, Republic of Korea) | Kim, D.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Cha, B.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Hwang, S.S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Yang, B.C. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Im, G.S. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Kim, M.J. (National Institute of Animal Science, RDA, Suwon, Republic of Korea) | Min, K.S. (Hankyong National University, Ansung, Republic of Korea) | Seong, H.H. (National Institute of Animal Science, RDA, Suwon, Republic of Korea), E-mail: [email protected]
DNA methylation is involved in tissue-specific gene control and essential for normal embryo development Octamer-binding transcription factor 4 (Oct-4) is one of the most important transcription factors for early differentiation. This study was performed whether the bovine Oct-4 is tissue specific or developmental dependent epigenetic mark, we investigated transcripts and the methylation status of CpGs of 5'-promoter region of Oct-4 in bovine preimplantation embryos. Oct-4 transcripts were highly detected in morula and blastocyst, while they were present low levels in sperm and 2- to 8-cell stage embryos. These results suggest that de novo expression of Oct-4 initiates at morula stage of embryogenesis. Here we determined that there is a tissue-dependent differentially methylated region (T-DMR) in the 5'-promoter region of Oct-4. The methylation status of the Oct-4 T-DMR was distinctively different in the oocyte from that in the sperm and adult somatic tissues and changed from zygote to blastocyst stage, suggesting that active methylation and demethylation occur during preimplantation development. Based on these results, the 5'-promoter region of Oct-4 gene is target for DNA methylation and the methylation status changes variously during embryonic development in bovine.
显示更多 [+] 显示较少 [-]