Genetic Transformation of Watermelon (Citrullus vulgaris Schard.) by Callus Induction
2007
Kwon, J.H. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea) | Park, S.M. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea) | Lim, M.Y. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea) | Shin, Y.S. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea) | Harn, C.H. (Nongwoo Bio Co., Ltd., Yeoju, Republic of Korea), E-mail: [email protected]
The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptII gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression.
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