Development of L-Threonine Producing Recombinant Escherichia Coli Using Metabolic Control Analysis
2007
Choi, J.I. (CJ Corp. RnD Center for Bioproduct, Seoul, Republic of Korea) | Park, Y.H. (CJ Corp. RnD Center for Bioproduct, Seoul, Republic of Korea) | Yang, Y.L. (CJ Corp. RnD Center for Bioproduct, Seoul, Republic of Korea), E-mail: [email protected]
New strain development strategy using kinetic models and metabolic control analysis was investigated. In this study, previously reported mathematical models describing the enzyme kinetics of intracellular threonine synthesis were modified for mutant threonine producer Escherichia coli TF5015. Using the modified models, metabolic control analysis was carried out to identify the rate limiting step by evaluating the flux control coefficient on the overall threonine synthesis flux exerted by individual enzymatic reactions. The result suggested the production of threonine could be enhanced most efficiently by increasing aspartate semialdehyde dehydrogenase (asd) activity of this strain.
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