Matrix metalloproteinases, interleukine-10, tumor necrosis factor-alpha, neuropeptids and apoptosis in cow endometrium in postparturition period | Matrices metaloproteināzes, interleikīns-10, tumoru nekrotiskais faktors-alpha, neiropeptīdi un apoptoze govju endometrijā pēcdzemdību periodā
2008
Sematovica, I., Latvia Univ. of Agriculture, Sigulda (Latvia). Agency Research Inst. of Biotechnology and Veterinary Medicine Sigra | Jemeljanovs, A., Latvia Univ. of Agriculture, Sigulda (Latvia). Agency Research Inst. of Biotechnology and Veterinary Medicine Sigra | Pilmane, M., Riga Stradins Univ. (Latvia). Faculty of Medicine. Inst. of Anatomy and Anthropology
Cow's uterus biopsy samples were taken in winter 2004/2005 at the research and training farm 'Vecauce'. Histological investigations were performed at the Institute of Anatomy and Anthropology of Riga Stradins University. Nine cows were biopsied twice: in the first and fifth week of postparturition. The aim of the research was to reveal inflammatory factors, neuropeptide-containing innervations, and apoptosis in the endometrium of cows in postparturition period. The results showed significant increase in the number of inflammatory cells, TNF-alpha, and apoptotic cells in endometrium between first and fifth week after parturition (p is less than 0.01). A mild positive correlation was found between the number of apoptotic cells and macrophages in endometrium (r= 0.54; p 0.1). In cow's endometrium, expression of MMP-2 was observed in macrophages, but it was not characteristic of processes in the cow's uterus in postparturition period. Compared to MMP-2 expression, MMP-9 expression was significantly higher (p is less than 0.05) and was characteristic of cow's uterus in postparturition. No statistically significant correlation was found between cells expressing IL-10, MMP-2, MMP-9, TNF-alpha, and PGP 9.5. A mild positive correlation (r=0.52; p is less than 0.05) between the number of TNF-alpha positive cells and infiltration of endometrium with inflammatory cells was detected. A mild positive correlation (r=0.58; p is less than 0.05) was observed between the number of TNF-alpha and apoptotic cells in the cows' endometrium. PGP 9.5 containing nerve fibbers appeared around endometrial glands; walls of blood vessels and in sub mucosal tissue and did not correlate with tissue infiltration by inflammatory cells, possibly due to the weakness of the immune system of individual animals.
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