Phylogenetic Characteristics of Viable but Nonculturable Bacterial Populations in a Pine Mushroom (Tricholoma matsutake) Forest Soil
2007
Kim, Y.J. (Mokwon University, Daejeon, Republic of Korea) | Whang, K.S. (Mokwon University, Daejeon, Republic of Korea), E-mail: [email protected]
The CFDA (6-carboxyfluorescein diacetate) direct viable count method and plate count (PC) method using conventional nutrient broth (NB) medium and 10-² diluted NB (DNB) medium were applied to samples collected from Mt. Yongdoo In Andong, in an effect to determine the number of living bacteria pine mushroom forest soil. The number of living bacteria determined via plate count in NB medium comprised 5~8% of the CFDA direct viable count, and the bacteria in the DNB medium comprised 40~47%. This result indicated that viable but nonculturable (VBNC) bacteria existed in the pine mushroom forest soil at a high percentage. The phylogenetic characteristics of the VBNC bacterial populations in the samples of pine mushroom (Tricholoma matsutake) forest soil were analyzed via the direct extraction of DNA and 16S rDNA-ARDRA.
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