Investigation of polyploidy in the breeding of lilies (Lilium spp.)
2008
Balode, A., Latvia Univ. of Agriculture, Jelgava (Latvia)
Some of best varieties of plants, especially ornamentals and fruits, were polyploid. Polyploids have larger flowers and stems than diploids. In the production of polyploid forms, different types of crosses were made. In present study a cross of diploid (2n = 2x = 24) and tetraploid (4n = 4x = 48) varieties resulted in the production of highly viable seeds 89.3% on average, but a cross of tetraploids and diploids – of 39.5% on average, with fluctuating results between cross combinations. Treating seeds and bulb scales with colchicine solutions can change chromosome number of 9 varieties. These results demonstrated that optimal concentration of colchicine was 1 g kgE-1 for chromosome doubling of seeds. A high concentration (2 g kgE-1) showed phytotoxicity that is detrimental for plant regeneration. The scales of bulbs treated with 1 g kgE-1 colchicine have resulted in the production of lily polyploid forms. The polyploid plants regenerated after pre-treatment by colchicine may be selected in early stage by phenotype of young bulblets. Broad, fleshy bulb scales setting right angle to growth axis were characteristic for regenerated tetraploids. The produced polyploid plants, in comparison with diploids, had a larger diameter of the flower (+2 up to 2.5 cm), more extended plant height (+10 up to 20 cm), increased flower count (+2) and was coming into bloom 4-10 days later than diploids of the same variety.
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