Micropropagation of high bush blueberry (Vaccinium corymbosum)
2008
Georgieva, M., Research Inst. of Mountain Stockbreeding and Agriculture, Troyan (Bulgaria) | Kondakova, V., Agro Bio Institute, Sofia (Bulgaria)
During the last years, the interest and production of high bush blueberry (Vaccinium corymbosum) increase a lot, especially in mountain region. It's one of the most significant berries with valuable characteristics and production of secondary metabolites as anthocyanin, which provide human health benefits. One of the limited factors for development and productions is the environmental conditions. High bush blueberry grows best and most commonly in moist or wet peat of moderate and high acidity soils. The utilization of biotechnology approaches for propagation of several berries cultivars (‘Bluecrop’, ‘Duke’, ‘Toro’ and ‘Brigitta Blue’) were study. The creation of an efficient in vitro system for regeneration was on the basal WPM medium. The effect of zeatin was compared with that of 2-iP in establishment and proliferation phases of leave and stem segments. Rooting of micropropagated plants and genotype relation were also studied. The best regeneration capacity of leave segments from cv. ‘Brigitta Blue’ (92.2%) was appear on WPM medium supplemented with zeatin - 4.10E-6 mg.lE-1 and 2-iP - 5.10E-6 mg.lE-1. The rooting under in vitro conditions was in 81.8% on 1/2 WPM medium supplemented with 10E-6 mg.lE-1 IAA for cv. ‘Brigitta Blue’.
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