Molecular characterization of introgression from Oryza longistaminata A. chev. et Roehr. into rice (Oryza sativa L.)
2007
Reintar, R.I.S., )
A set of 33 advanced alien introgression lines (AILs) derived from the cross of rice (O. sativa) and a diploid wild species, O. longistaminata was evaluated for yield and yield components. Significant differences were found for different agronomic traits. Although, the donor parent, O. longistaminata, is phenotypically inferior however, transgressive segregants were obtained for yield and yield components. Two alien introgression lines outperformed the recurrent parent in terms of yield. Three high yielding alien introgression lines (AILs 8, 16, 21) carried O. longistaminata alleles as detected through RM530, RM8255 and RM122 in the long arm of chromosome 2 where yld 2.1 QTl for yield are located. O. longistaminata alleles were found on chromosomes 6 and 7 of the AILs having increased number of tillers per plant and panicle length, respectively. These AILs may carry favorable alleles from wild species that would contribute to higher yield. A total of 237 polymorphic SSR markers were used to detect introgression from O. longistaminata into O. sativa. Some of the AILs did not show any introgression. On an average each AILs had 3-29 introgressed segments with an average of 12 segments from the O. longistaminata. Presence of non-parental bands was observed in some of the AILs in every chromosome. SSR analysis of chromosome segmental substitution lines (CSSL) revealed 0.10 O. longistaminata segments per individual chromosomes. The size of the introgressed segments ranged from 0-8.1 cM genetic length.
显示更多 [+] 显示较少 [-]