DNA fingerprinting of wheat (Triticum aestivum L.) genotypes as revealed by random amplified polymorphic DNA (RAPD) analysis
2006
Ahmed, A. (University of Arid Agriculture, Rawalpindi (Pakistan). Dept. of Biochemistry) | Ahmed, K. (University of Arid Agriculture, Rawalpindi (Pakistan). Dept. of Biochemistry) | Gulfaraz, M. (University of Arid Agriculture, Rawalpindi (Pakistan). Dept. of Biochemistry) | Kisana, N.S. (National Agricultural Research Centre, Islamabad (Pakistan)) | Asim, M. (National Agricultural Research Centre, Islamabad (Pakistan))
A study was conducted to assess the genetic diversity of Wheat (Triticum aestivum L.) by DNA fingerprinting by Random amplified polymorphic DNA (RAPD) analysis. A total of 44 wheat accessions representing local landrace genotypes, candidate lines and few commercial varieties were evaluated by using (RAPD) while DNA was extracted from wheat leaves and was quantified which was further subjected to PCR analysis with the help of different primers. The primers varied in their GC content, having an arbitrary sequence. The products obtained after PCR analysis were visualized using 0.9% Agarose gel. In electrophoresis, results showed appreciable amount of genetic diversity in the germplasm under study. Further more such a stud; also, represented the usefulness of this technique used to investigate the relationship based on the RAPD analysis.
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