Nutritional requirements for laboratory cultivation of the cyanobacterium, Microcystis aeruginosa (Cyanophyta) isolated from Laguna de Bay, Philippines
2006
Sese, M.L.D. | Goss, M.R.M.
Microcystis aeruginosa, a bloom-forming cyanobacterium isolated from Laguna de Bay, Philippines, was cultivated in the laboratory with the objectives of establishing the suitable culture medium for its growth and determining the most likely nutrient responsible for its optimum growth under laboratory, and possibly, a natural conditions. Among the various culture media tested, it was observed that M. aeruginosa grew best in Tris-buffered inorganic medium (TBIM) probably due to its high nitrogen content and good buffering capacity. Varying levels of nitrogen in this medium revealed that twice its recommended concentration (approximately 56 ppm N) gave the optimum microalgal growth. In this medium nitrogen content, phosphorous at 1.5 to 3 times the recommended concentration (approximately 46 to 93 ppm P) showed the highest microalgal growth. Supplying nitrogen in the medium from different commercial inorganic fertilizer sources showed that ammonium sulfate gave the best result for the growth of the microalgae in the laboratory. Chemical analysis of the field-cultured cyanobacterium revealed high nitrogen content (average = 30.5%) and moderate amounts of phosphorous (average = 0.75%) and potassium (average = 1.95%). Results of this study seem to indicate that the high nitrogen and phosphorous requirement of M. aeruginosa may provide an explanation for its bloom-forming capacity in most eutrophic lakes.
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