High production of wheat double haploids via anther culture
2007
Kondic-Spika, A., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Kobiljski, B., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Hristov, N., Institute of Field and Vegetable Crops, Novi Sad (Serbia)
Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and the lowest values for these traits (9 and 21%, respectively). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breeding programs for rapid production of homozygous wheat lines.
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