Identification of Glu-B1 alleles in bread wheat cultivars using PCR
2007
Obreht, D., Faculty of Sciences, Novi Sad (Serbia). Department of Biology and Ecology | Kobiljski, B., Institute of Field and Vegetable Crops, Novi Sad (Serbia) | Djan, M., Faculty of Sciences, Novi Sad (Serbia). Department of Biology and Ecology | Vapa, Lj., Faculty of Sciences, Novi Sad (Serbia). Department of Biology and Ecology
In order to implement recently developed PCR assay for Glu-B1 x-type allele discrimination in marker-assisted selection, a set of wheat cultivars from 11 countries was analyzed. The PCR results matched the known Glu-B1 HMG GS genotypes of the cultivars possessing subunits Bx7 or Bx17. It was shown that this molecular marker also diferentiated some other Glu-B1 alleles: Bx6, Bx13, Bx20 and Bx22. These results create the opportunity for an improved method of polymorphism scoring at the Glu-B1 in bread wheat germplasm.
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