Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surface
2009
Milanov, D., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Asanin, R., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine | Vidic, B., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Krnjaic, D., University of Belgrade, Belgrade (Serbia). Faculty of Veterinary Medicine | Petrovic, J., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Savic, S., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)
Listeria monocytogenes is the causative agent of numerous endemics and sporadic cases of illness in humans. Food is principal route of infection. Although it appears to be widely accepted that Listeria monocytogenes forms biofilms on food-processing surfaces, there has been very little direct microscopical evidence to support this. In this study we investigated the ability of biofilm formation in 14 strains of Listeria monocytogenes of different origin. The biofilms were formed on stainless steel surfaces during 7 days of incubation in a tryptone soy broth supplemented with 0.6% yeast extract (TSB-YE) at a temperature of 25 deg C. The developed structures were examined using scanning electron microscopy. Our results revealed significant differences among tested Listeria monocytogenes strains in terms of their ability to form a biofilm in given experimental conditions. All strains proved weak producers of extracellular polymeric substance.
显示更多 [+] 显示较少 [-]