Investigations of hemadsorption characteristics of glycoprotein HN and F antigen of parainfluenza 3 virus outer membrane in vitro | Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljasnjeg omotaca virusa parainfluence 3, in vitro
2006
Nisavic, J., Fakultet veterinarske medicine, Beograd (Serbia). Katedra za mikrobiologiju | Milic, N., Fakultet veterinarske medicine, Beograd (Serbia). Katedra za mikrobiologiju
The objective of the investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the outer membrane of the parainfluenza 3 virus. Six hours following inoculation of the cell line with the Vero virus PI3, previously activated with 0.025 g/dl trypsin-versen, the appearance of less intense hemadsorption was established in antigen dilutions of 1:2 and 1:4. Hemadsorption was established in the mentioned cell lines also 12 h after inoculation in virus dilutions of 1:8 and 1:16, while 24 h following inoculation, hemadsorption was present also in a virus dilution of 1:64. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:16 was established 24 h after inoculation of the cell line with Vero samples of the virus PI3, activated with trypsin-versen and with the addition of specific immune serum.
显示更多 [+] 显示较少 [-]