Appearance of fungus Screrotinia sclerotiorum (Lib.) de Bary on soybean seed in the period from 2005 to 2007 | Pojava gljive Sclerotinia sclerotiorum (Lib.) de Bary na semenu soje u periodu od 2005. do 2007. godine
2009
Petrovic, D. | Ignjatov, M. | Vujakovic, M. | Taski-Ajdukovic, K. | Nikolic, Z., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Milosevic, M., Ministarstvo poljoprivrede, sumarstva i vodoprivrede Republike Srbije, Beograd (Serbia)
Sclerotinia sclerotiorum causes white rot disease in plants and has a very wide range of hosts. Some 30 individual soybean samples were included in our investigation and the average level of seed infection at different localities in the Vojvodina Province (Serbia): Vrbas, Backa Palanka, Srbobran, Rimski Sancevi during 2005, 2006, and 2007 was determined. Isolation, identification and determination of Sclerotinia sclerotiorum were done on the PDA nutrient medium according to the NSHS Sf 3.1. method. Seed incubation was done for 10 days at 25 deg C, and infection was estimated after 3, 5, 7, and 10 days. The fungus develops characteristic and easily recognized colonies. The fungus mycelia are white and cream in colour, and the sclerotia develop regularly, mostly at the edges of the colony. Our results showed relatively low levels infection by fungus, with the average levels over the years being 0.25-3.25% in 2005, 0.25-1.75% in 2006, and 1.50-2.5% in 2007.
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