Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
2008
Bozic, N. | Dojnov, B. | Milovanovic, A., Institute of Chemistry, Tehnology and Metallurgy, Belgrade (Serbia). Center of Chemistry | Nenadovic, V. | Ivanovic, J., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Insect Physiology and Biochemistry | Vujcic, Z., University of Belgrade, Belgrade (Serbia). Faculty of Chemistry
Application of chomogenic substrates, use of class-specific inhibitors, and zymogram analysis enabled us to identify several peptidase classes in extracts of the midgut of Morimus funereus larvae. Zymogram analysis with gelatin as a peptidase substrate and phenylmethylsulfonyl fluoride as an inhibitor showed that serine peptidases were the most abundant endopeptidases in the midgut of M. funereus larvae. By zymogram analysis with gelatin as a peptidase substrate and 1,10-phenantroline as an inhibitor, metallopeptidases were also detected. Analyses of serine peptidases with specific chromogenic substrates revealed dominance of elastase-like peptidases in extracts of the midgut of M. funereus larvae, with less pronounced chymotrypsin- and trypsyn-like activities.
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