Dynamic associations of transcription factors with the rat liver nuclear matrix are functionally related to differential alfa-2-macroglobulin gene expression
2008
Dinic, S. | Mihailovic, M. | Ivanovic-Matic, S. | Uskokovic, A. | Grdovic, N. | Vidakovic, M. | Poznanovic, G., Institute for Biological Research Sinisa Stankovic, Belgrade (Serbia). Department of Molecular Biology
Participation of the nuclear matrix in regulation of alfa-2-macroglobulin (alfa2M) gene transcription during rat liver development and the acute-phase (AP) response are examined. DNA affinity chromatography of fetal and adult liver internal nuclear matrix proteins under basal and AP conditions with the alfa2M gene promotor (-852/+12) and immunoblot analysis revealed diverse patterns of association of transcription factors with the nuclear matrix. HNF-6, C/EBPalfa, and STAT5b were involved in basal and C/EBPbeta, STAT1, and STAT3 in AP-stimulated alfa2M expression. These findings support the assuption that transcription factor-nuclear matrix interactions serve to channel gene regulatory proteins to DNA sequences.
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