Coriolus hirsutus laccase effect on atrazine adsorption and desorption by different types of soil
2008
Davidchik, V.N. | Golubeva, L.I. | Stepanova, E.V. | Koroleva, O.V., Russian Academy of Sciences, Moscow (Russian Federation). The A.N. Bakh Biochemistry Inst. | Kulikova, N.A., Lomonosov Moscow State Univ. (Russian Federation)
The objects under study were samples of sod-podzolic soil, forest grey soil and black soil. The samples were collected from a 0-5 cm layer. Adsorption and desorption experiments were carried out with solutions atrazine in concentrations of 1, 2, 3, 5, 8 and 10 milli g/l. The concentration of laccase produced by soil fungus Coriolus hirsutus was 3.5 g/l. In the no application laccase the shape of isotherms was close to linear. In the presence of the enzyme at the beginning there was an abrupt rise of adsorption of atrazine, but in course of time the adsorption decreased. The Freundlich adsorbtion constants for sod-podzolic soil grew from 4.50 to 5.80, for forest grey soil – from 0.81 to 3.13, for chernozem – from 5.55 to 6.79. Partial reversibility of atrazine adsorption reducing in the presence of laccase was found out. An increase in the concentration of atrazine in the medium did not reduce the reversibility of its adsorption with soils. Supposedly, atrazine binding was oxidative but was not provided by only physical adsorption. The results obtained can be used to develop new detoxication technologies of soils polluted with atrazine and other herbicides
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