The study of the nonpathogenic influenza virus A/gull/Moscow/3100/2006 (H6N2) isolated in Moscow
2009
Gambaryan, A.S. | Boravleva, E.Yu. | Kropotkina, E.A., Russian Academy of Medical Sciences, Moscow Region (Russian Fedeartion). The M.P. Chumakov Inst. of Poliomyelitis and Viral Encephalitis | Lomakina, N.F., The Ya.R. Kovalenko All-Russia Research and Development Inst. of Experimental Veterinary, Moscow (Russian Federation) | Kirillov, I.M. | Lavrentiev, M.V. | Yamnikova, S.S., Russian Academy of Medical Sciences, Moscow (Russian Federation). The D.I. Ivanovskij Research and Development Inst. of Virology
In autumn 2006 during an ecological monitoring of water bodies in Moscow from gull feces within the precincts of Moscow the hemagglutinating agent was isolated. It was identified as the influenza virus A/H6N2/ and called A/gull/Moscow/3100/2006. The determination of the primary structure of the complete virus genome (GenBank, EV152234-EV152241) allowed finding out its genotype: K, G, D, 6B, F, 2D, F, 1E having no analog in the International Database GenBank. Analyzing individual genes of the A/gull/Moscow/3100/2006 (H6N2) strain has shown that its genome has been formed as a result of specific host succession and multiple gene exchange between viruses spread in the territories of Italy, Netherlands, Denmark and Germany in 1999-2006. As is found, the A/gull/Moscow/3100/2006strain is not pathogenic, it does not cause death of 9-11-day-old chicken embryos and mice inducing in the latter produci8ng specific antibodies. The receptor phenotype is typical for gull influenza viruses: the virus exclusively recognizes receptors with Neu5Ac(alpha)2-3 Gal that is characteristic of avian influenza viruses manifesting high affinity to fucosylated sialosugars in contrast to duck viruses accompanied with restructuring amino acids in the hemagglutinin loop. A characteristic feature of the A/gull/Moscow/3100/2006 strain is the presence of amino acids Ser 224 and Trp 229. it is concluded that the influenza virus A/gull/Moscow/3100/2006 isolated in Moscow has no famous pathogenicity markers. By the receptor specificity it is intermediate between duck viruses and gull viruses. The low affinity to the receptors typical for human viruses says that the influenza virus A/gull/Moscow/3100/2006 is unable to propagate in the mammal organisms without preliminary adaptation
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