Photoheterotrophic callus culture Ficus elastica, the formation of polyisoprene synthesis ability
2009
Kolchugina, L.B. | Markarova, E.N., Lomonosov Moscow State Univ. (Russian Federation)
The ability to synthesize polyisoprene was studied for lines of photoheterotrophic callus cul¬ture Ficus elastica, which result from the selection and prolonged cultivation of the culture in light in media containing 1% sucrose in the presence of 0,5 and 0,05 mg/1 BAP and in the absence of the hormone. Results of microscopic studies show that the F. elastica lines are able to synthesize polyisoprene typical of intact plants. The substantial accumulation of polyisoprene was observed for the culture grown in the BAP-free media. Moreover, for some lines considered, tracheide structures were shown to be present in tissues. The formation the tracheide structures correlates directly with the polyisoprene synthesis. We conclude that the changes in the BAP content in the cultivation medium and selection allow the culture exhibiting a certain functional specificity to be prepared. Under optimum cultivation conditions, the culture develops toward the intensification the polyisoprene synthesis
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