COMPARATIVE GENDER-RELATED ANALYSIS OF THE LOCALIZATION OF TISSUE ALKALINE AND ACID PHOSPHATASE EXPRESSION IN THE URETHRA IN DOMESTIC CATS (FELIS SILVESTRIS CATUS)
2010
Dimitrov, R., Thracian University, Stara Zagora (Bulgaria) | Vonkova, P., Thracian University, Stara Zagora (Bulgaria) | Vladova, D., Thracian University, Stara Zagora (Bulgaria) | Kostov, D., Thracian University, Stara Zagora (Bulgaria) | Stefanov, M., Thracian University, Stara Zagora (Bulgaria)
AIM: Detection of the presence of acid and alkaline phosphatase, whose tissue expression indicates the course of normal and pathological events in the urethra, as well as gender-related peculiarities in the activities of studied enzymes in this anatomical area. MATERIAL AND METHODS: The pelvic urethra from six male cats and the urethra of six female cats, all clinically healthy and sexually mature. The expression of acid and alkaline phosphatase was detected by Gomori staining. RESULTS: The enzyme histological investigation of pelvic urethra in male cats showed that the most significant localization of acid phosphates activity was observed in the propria and adventitia of pelvic urethra, followed by apical parts of epithelial cells in disseminated part of the prostate and in the lumen of glandular tubules. In the lumen of disseminated prostatic alveoles, no alkaline phosphatase activity was detected. In female cats, the strongenst acid phosphatase activity was established in the connective tissue located among skeletal muscle fibres, followed by a slighly detectable enzyme expression in the propria and perivascular areas of the urethra, whereas alkaline phosphatase activity was present in the connective tissue among skeletal muscle fibres. In the other connective tissue elements of the urethra and its epithelium, female cats exhibited no alkaline phosphatase activity. CONCLUSION: The lack of tissue alkaline and acid phosphatase activity in urethral propria in female cats was considered.
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