Determination of glyphosate in soil by high performance liquid chromatography after derivatization with p-toluenesulphonyl chloride | 对甲基苯磺酰氯衍生化液相色谱法测定土壤中草甘膦
2009
Si Youbin, Anhui Academy of Agricultural Science, Hefei(China), School of Resources and Environment | Sang Zhongying, Anhui Academy of Agricultural Science, Hefei(China), School of Resources and Environment | Cheng Fengxia, Anhui Academy of Agricultural Science, Hefei(China), School of Resources and Environment
中国人. 建立简单、灵敏的土壤中草甘膦残留量的高效液相色谱检测方法。土壤样品用0．6mol・L. 1KOH提取，经对甲基苯磺酰氯衍生化后，注入Cj8柱中分离，用紫外检测器在235FIB波长检测，流动相组成为0．05mol・L. 1磷酸盐缓冲液（pH5．5）：甲醇=65：35（V：V）。样品中苹甘膦浓度在0．5～40mg・L. 1范围内与峰面积呈线性关系（R. =0．9998）。草甘膦的最小检出量（LOD）为2×10. 10g，土壤中最低检出浓度（LOQ）为0．02mg・kg. 1，平均回收率及变异系数分别为81．3％-98．3％和0．66％～5．63％。[著者文摘]
显示更多 [+] 显示较少 [-]英语. A simple, sensitive and selective method using high performance liquid chromatography （HPLC） was developed to determine residues of glyphosate in soil. The soil sample was extracted with 0.6 mol・L. 1 KOH, and glyphosate in solution was derivatized with p- toluenesulfonyl chloride under alkaline conditions. An aliquot of the reaction mixture was injected into a C18-column with a mobile phase consisting of 0. 05 mol・L. 1 phosphate buffer （ pH 5.5 ） -methanol （65 ： 35, V/V） and detected at 235 nm. The response was linear in the range 0. 5 - 40 mg・L. 1 for glyphosate in the sample solution （R. = 0. 999 8）. The limit of detection was estimated to be 2 × 10. 10 g, and the minimum determination concentration of glyphosate in soil samples was 0. 02 mg・L. 1. The ranges of average recoveries and coefficient variation of the method were 81.3% -98.3% and 0. 66% -5.63% ,respectively.
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