Determination of estrogen residues in milk by HPLC | 牛乳中三种雌激素残留的HPLC检测法
2009
Zhao Jinlian, Gansu Agricultural University,Lanzhou(China) | Du Lanxiang, The Center of Control of Disease in Anning District,Lanzhou(China) | Li Yuejuan, The Center of Inspection and Monitoring of Production Quality in Gansu Province,Lanzhou(China)
中国人. 建立了牛奶中3种雌激素残留量（雌二醇、己烯雌酚、雌酮）的HPLC（高效液相色谱）检测方法.得到色谱条件为流动相：乙腈-水（体积比为1∶1）;检测波长：230 nm;流速1.0 mL・min. 1.采用该方法测定了市售鲜牛奶中3种雌激素的残留量,结果表明：其分离效果良好,平均回收率范围82.2%-95.5%,相对标准偏差为2.0%-4.7%,最低检出限达1.0-2.0 ng・mL. 1.该方法具有灵敏、准确、精密、无杂质干扰等优点.[著者文摘]
显示更多 [+] 显示较少 [-]英语. To study an efficient method for dertermination of three estrogens（estradiol,diethylstilbestrol and norethisterone） in milk,the HPLC was developed by using acetonitrile,the supernatant from the samples with the protein precipitated and removed was concentrated using HPLC,acetonitrile-water（volume ratio of 1∶1） as mobile phase for analysis.The results indicated that good separation was achieved in 3 components and the recovery rate was between 82.2 % and 95.5 %,the relative standard deviation was between 2.0 % and 4.7 %,the lowest detection limit was between 1.0 ng・mL. 1 and 2.0 ng・mL. 1.The method was proved to be satisfactory in precision,acuracy and sensitivity.
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