Microsatellite DNA analysis on genetic diversity in five sheep breeds | 五个绵羊品种遗传多样性的微卫星分析

中国人. 利用8个微卫星标记对5个绵羊品种（藏羊、兰州大尾羊、蒙古羊、小尾寒羊、无角道赛特）进行了遗传多样性分析.结果表明：5个绵羊群体的8个微卫星位点共发现105个等位基因,平均每个位点有13个,其中,在OarFCB128位点检测到的等位基因数最多,在BM8125位点检测到的等位基因数最少.多态信息含量、有效等位基因数及群体杂合度数据表明,小尾寒羊遗传变异最大,其次是蒙古羊、兰州大尾羊、藏羊,无角道赛特变异最小.根据Nei氏遗传距离用NJ法和UPGMA法构建的聚类图表明,兰州大尾羊与蒙古羊聚为一类,遗传距离最近,与无角道赛特距离最远.[著者文摘]

英语. The genetic diversity in 5 sheep breeds（Tibet sheep,Lanzhou large-tailed sheep,Mongolia sheep,Small-tailed Han sheep and Poll Dorset sheep） was analyzed by using 8 microsatellite DNA markers.The results indicated that,105 alleles for 8 microsatellite loci in 5 sheep breeds were discovered,the average allele number on a locus was 13.There were the most number of alleles in OarFCB128,and the least in BM8125.The data of polymorphism information contents（PIC）,effective number of alleles（Ne） and heterozygosity（He） indicated that,the genetic variation of Small-tailed Han sheep was the highest,Mongolia sheep was the second,Lanzhou large-tailed sheep was the third,Tibet sheep was the fourth,Poll Dorset sheep was the lowest.According to the Nei genetic distances,the clustering figures were established through the NJ tree and UPGMA tree,the results showed that Lanzhou large-tailed sheep and Mongolia sheep were clustered together,whose genetic distances was the nearest,the genetic distances between Lanzhou large-tailed sheep and Poll Dorset sheep was the farthest.