Classification and identification of marine penicillium species based on ITS sequences of rDNA | 利用ITS核酸序列分析鉴定海洋青霉菌
2009
Qu Lingyun, First Institute of Oceanography, SOA, Qingdao (China) | Tian Li, First Institute of Oceanography, SOA, Qingdao (China) | Sun Xiuqin, First Institute of Oceanography, SOA, Qingdao (China)
[Objective] The aim of this study is to identify 6 marine fungi species by analyzing ITS nucleotide sequence, which had been primarily identified as penicillium based on morphological characteristics. [Method] The ITS regions of these species were cloned by molecule biology method and phylogenetic analyzed using ClustalX1.83 software. [Result] The ITS regions of these species were sequenced, and phylogenetic analysis between the yield sequences and the ITS sequences assessed in GenBank showed that the 6 strains all belonged to penicillium. [Conclusion] The present study suggests ITS sequence analysis could not be used as an only proof, but it is a very useful supplementary tool for the classification and identification of marine penicillium combined with morphological characteristics.
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