Waterlogging induced oxidative stress and antioxidant enzyme activities in pigeon pea
2009
Sairam, R.K.,Indian Agricultural Research Inst., New Delhi (India). Div. of Plant Physiology | Kumutha, D.,Indian Agricultural Research Inst., New Delhi (India). Div. of Plant Physiology | Ezhilmathi, K.,Indian Agricultural Research Inst., New Delhi (India). Div. of Plant Physiology | Chinnusamy, V.,Indian Agricultural Research Inst., New Delhi (India). Div. of Plant Physiology | Meena, R.C.,Indian Agricultural Research Inst., New Delhi (India). Div. of Plant Physiology
An experiment was conducted with two contrasting pigeon pea genotypes, ICPL 84023 (tolerant) and ICP 7035 (susceptible), to study the physiological and molecular basis of waterlogging tolerance in relation to oxidative stress and antioxidant enzyme activities. Waterlogging resulted in visible yellowing and premature senescence of leaves, and greater decline in relative water content, chlorophyll content, and membrane stability index in ICP 7035 than in ICPL 84023. Superoxide radical and hydrogen peroxide contents increased at day 4 and 6 of waterlogging probably due to activation of NADPH-oxidase. The expression of NADPH oxidase-mRNA was increased under waterlogging condition in ICPL 84023. ICP 7035 showed higher contents of ROS in control conditions and after recovery, however, during waterlogging the superoxide radical production was higher in ICPL 84023. Activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase and catalase increased under waterlogging more in ICPL 84023 than in ICP 7035. Cu/Zn-SOD and APX-mRNA expression in 24-h waterlogged plants showed enhanced expression in ICPL 84023 compared to ICP 7035. The cloning and sequencing of APX gene of tolerant and susceptible genotypes yielded cDNAs of 622 and 623 bp, having 95 % homology with each other and 92 % with the corresponding sequences of Vigna unguiculate APX-gene.
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