Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs
2009
Vykoukalova, Z.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Knoll, A.,Mendelova Zemedelska a Lesnicka Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Cepica, S.,Akademie Ved, Libechov (Czech Republic). Ustav Zivocisne Fyziologie a Genetiky
Perilipin, encoded by the PLIN gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine PLIN gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan x Pietrain cross. Associations were studied between loci g.4119A G and g.7966T C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A G and g.7966T C only genotype combinations AA-TT, AG-TC and GG-CC were detected. A significant difference (P = 0.0290) between GG-CC and AG-TC genotype combinations for BFT in herd 2 and a suggestive difference (P = 0.0516) between GG-CC and AA-TT genotypes for ADG in herd 1 were detected.
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