Transformation of chickpea: effect of genotype, explant, Agrobacterium-strain and composition of culture medium
2010
Bhattacharjee, B.,International Centre for Genetic Engineering and Biotechnology, New Delhi (India) | Mohan, M.,International Centre for Genetic Engineering and Biotechnology, New Delhi (India) | Nair, S.,International Centre for Genetic Engineering and Biotechnology, New Delhi (India)
Reproducible and high-frequency transgenic plant regeneration from callus and embryo axes of four different genotypes of Cicer arietinum was achieved after Agrobacterium-mediated transformation. Three different strains of Agrobacterium (EHA105, AGL1 and LBA4404) harboring the binary vector pCAMBIA1301 containing beta-glucuronidase (GUS) and hygromycin phosphotransferase (hpt) genes under the control of a CaMV35S promoter were used. The highest number of transgenic plants was obtained from cotyledonary node-derived calli of genotype Pusa-256. A highly efficient rooting was achieved on Murashige and Skoog medium supplemented with indole-3-butyric acid. The stable integration of the gene was confirmed by molecular analyses of the transformed plants. Inheritance of GUS and hpt gene was followed through two generations and they showed the expected 3:1 inheritance.
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