Direct somatic embryogenesis and plant regeneration from immature explants of chickpea
2010
Kiran Ghanti, S.,Gulbarga Univ. (India). Dept. of Botany | Sujata, K.G.,Gulbarga Univ. (India). Dept. of Botany | Srinath Rao, M.,Gulbarga Univ. (India). Dept. of Botany | Kavi Kishor, P.B.,Osmania Univ., Hyderabad (India). Dept. of Genetics
A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea cultivars, ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog's (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), alpha-naphthaleneacetic acid (NAA) and picloram alone or in combination with 0.5 - 2.0 mg/cubic dm N6-benzylaminopurine (BA) or kinetin (KIN). NAA was better for somatic embryo induction compared to other auxins. The well formed, cotyledonary shaped embryos germinated into plantlets with 36.6 % frequency on MS medium supplemented with 2.0 mg/cubic dm BA + 0.5 mg/cubic dm abscisic acid (ABA). The frequency of embryogenesis and plantlet regeneration was higher in cv. ICCV-10 than in cv. Annigeri. Regenerated plants were transferred to soil (40 % survival) and grown to maturity. Histological studies of explants at various developmental stages of somatic embryogenesis revealed that somatic embryos developed directly from the cotyledon cells and they were single cell origin.
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