Mitochondrial and Y chromosome DNA variability in Parda de Montaña breed | Variabilidad del ADN mitocondrial y del cromosoma Y en la raza bovina Parda de Montaña
2009
Calvo, J.H., Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza (España) | Sanz, A., Universidad de Zaragoza (España). Facultad de Veterinaria | Blanco, M., Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza (España) | Zaragoza, P., Universidad de Zaragoza (España). Facultad de Veterinaria | Revilla, R., Gobierno de Aragón, Zaragoza (España). Centro de Transferencia Agroalimentaria | Villalba, D., Universitat de Lleida (España). Escola Técnica Superior d'Enginyeria Agrària | Rodellar, C., Universidad de Zaragoza (España). Facultad de Veterinaria | Casasús, I., Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza (España)
We have studied the genetic variability and relationship of eight populations of Parda de Montaña breed using mitochondrial DNA and four Y chromosome microsatellites (BM861, BYM-1, INRA124 and INRA189). Y chromosome microsatellites showed monomorphics. Thirty-three mitochondrial haplotypes defined by 31 polymorphic sites (fourteen polymorphic sites were singletons) were found. The 17 phylogenetically informative sites were used to calculate nucleotide diversity (Pi=0.006) and haplotype diversity (Hd=0.890). A total of 16 previously undescribed haplotypes were found. The analysis of molecular variance (AMOVA) indicated that 10.4% of the genetic diversity was attributable to differences among populations. Fu's Fs (-35.008; p<0.0001), Fu and Li's F and Tajima's D (- 2.11204; p<0.05) indicated a clear deviation from the expected allele frequency spectrum, in this case due to an excess of rare alleles. The construction of median networks showed a clear star-like phylogeny around the most frequent haplotype. The negative Tajima's D, Fu and Li's F and Fu's Fs values, as well as expected mismatch distributions and median networks suggest a population expansion.
显示更多 [+] 显示较少 [-]