Beta-N-acetyl-glucosaminidase decreases the spermatozoa-zona pellucida and in vitro penetration in pig | La beta-N-acetil-glucosaminidasa disminuye la unión espermatozoide-zona pelúcida y la penetración in vitro en la especie porcina
2009
Coy, P., Universidad de Murcia (España). Facultad de Veterinaria | Ondiz, A. de, Universidad de Murcia (España). Facultad de Veterinaria | Romar, R., Universidad de Murcia (España). Facultad de Veterinaria
In mammals, sperm_egg interactions are mediated by protein receptors on the sperm plasma membrane attaching to carbohydrate containing molecules on the oocyte. Glycosidases are enzymes catalyzing hydrolytic cleavage of terminal sugar residues from glycoproteins. So, they could play a role in the modification of sperm receptors on the oocyte thus reducing the number of sperm attached and their further penetration into the ooplasm. We have reported activity for five exoglycosidades in the bovine and porcine oviductal fluid. In this study we evaluated the role of one of these glycosidases on the sperm-ZP binding and penetration in porcine oocytes. In vitro matured oocytes were partially denuded and incubated for 30 min with (0.5 micro l/ml) or without beta-N-acetyl-glucosaminidase. Then, oocytes were gently washed and inseminated at 2 x 10E5 spermatozoa/ml. After 10 min of coculture, oocytes were washed to remove the excess of sperm attached to the ZP and transferred to fresh TALP medium. Fertilization results were assessed at 18 h postinsemination. Incubation of oocytes with beta-N-acetyl-glucosaminidase decreased the number of sperm attached to the ZP and the mean number of sperm per penetrated oocyte, so increasing monospermy rates.
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