Effect of cryopreservation on integrity of plasmatic and acrosomal membrane of bulls sperm | Efecto de la criopreservación sobre la integridad de la membrana plasmática y acrosomal de espermatozoides de toros
2009
Rubio Guillén, J. | Quintero Moreno, A. | Osorio Meléndez, C. | González Villalobos, D.
Plasmatic and acrosomal membrane integrity has been used as valuable information for determining sperm quality, because is important to know the morphological and functional role as cellular delimitation and in effective cell interactions. The aim of this study was to determine cryopreservation effects on vitality (VIT), number of damaged acrosomes (DAR) and functional sperm plasma membrane integrity of bulls. The functional integrity of membrane was evaluated by osmotic tests (HOST and ORT). In all cases, there were used 5 fresh ejaculates and 5 thawed straws. The data was analysed by GLM procedure, and when differences were detected, LSMEANS was used to quantify the effects. Significant differences were found on seminal quality parameters (VIT, DAR, ORT and HOST) between fresh and thawed sperm. Freezing-thawing procedure had detrimental effect on the integrity structural and functional of MP and MA in the spermatozoa evaluated (P<0.05). However, it was possible to demonstrate that the spermatozoa have different pattern of resistance to the detrimental effects of cryopreservation. Then, this study confirms that cryodamage could happen indistinctly on the structural and functional MP and MA integrity, which would affect the fertilizing capacity of seminal samples destined for Artificial Insemination.
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