Loop-mediated isothermal amplification of DNA (Lamp): A Novel tool for diagnosis.
2009
Joshi, R.K. | Joshi, Namita | Shukla, Vivek Kumar | Niyogi, D. | Singh, Y. Damodar | Mukhopadgayay, S.K.
Nucleic acid amplification technologies are used in the field of molecular biology and recombinant DNA technologies. These techniques are used as leading methods in detecting and analyzing a small quantity of nucleic acid. The polymerase chain reaction (PCR) is the most widely used method for DNA amplification for detection and identification of infectious diseases, genetic disorders and other research purpose. However, it requires a thermo cycling machine to separate two DNA strands and then amplify the required fragment. Novel developments in molecular biology of DNA synthesis in vivo demonstrate the possibility of amplifying DNA in isothermal conditions without the need of a thermocycling apparatus. DNA polymerase replicates DNA with the aid of various accessory proteins. Recent identification of these proteins has enable development of new in vitro isothermal DNA amplification methods mimicking these in vivo mechanisms.
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