Differentiation of nucellar and zygotic seedling in citrus using ISSR markers.
2009
Shareefa, M | Singh, A K | Srivastav, Manish | Dubey, A K
In the present study we propose the use if inter simple sequence repeat (ISSR) markers as an alternate methodology for early differentiation of zygotic and nucellar seedling in circus. The DNA amplification profile of in vitro grown seddling was compared to that of respective mother plant. Nucellar seedling displayed a similar banding pattern as that of mother plants, here's zygotic seedling were differentiated from other seedling by their different banding pattern. The primers P-7, I-9, I-10, I-13 and I-14 were able to identify the zygotic seedling based on polymorphic bans in Soh sarkar. IN Attani zygotic seedlings were identified using the primers _-1, P-7, P-15, I-8, I-9, I-10, I-13 and I-14. out of 8 primers tested only 2 primers I-8 and I-14 showed polymorphism in kinnow and could differentiate zygotic seedlings. The primer P-1, I-8, I-9, I-14 and P-15 generated polymorphism among Karna khatta seedlings enabling distinction between nuceller and zygotic seedlings. In most of the cases single ISSR primer was not able to identify all the zygotic seedlings. However, a combination of ISSR primers identified almost all the zygotic seedling.
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