Liquid Chromatography method for Estimation of free Amino acids and a Growth Regulator from Chick pea and Tomato infected with Meloidogyne incognita
2010
Meher, H.C. | Gajbhiye, V.T. | Prasad, D. | Singh, G.
A simple and rapid Liquid Chromatography method for estimation of histidine, methionine, leucine, isoleucine, phenylalanine, tyrosine, tryptophan and gibberellic acid from Cicer arietinum and Solanum lycopersicum was used. The main advantage is that it did not require sample derivatization and other amino acids did not interfere in the analysis. The method was based on a binary gradient resolution of the analytes in a reverse phase C18 column and UV detection and estimation at a ë (wavelength) of 210 nm. The mobile phase comprised of A [water + TFA (0.1)] and B [acetonitrile + TFA (0.085)]. The gradient was 25 B in 25 min at a flow rate of 1 ml /min with an equilibration time of 15 min. The method easily measured Phe, Tyr and Trp implicated in nematode-plant interactions. The method was validated for specificity, linearity, precision and applied for estimation of the test analytes from root, shoot and fruit of chick pea and tomato. The concentration of amino acids and GA3 were more in the galled than un-galled root tissues and consequently, their levels decreased in shoots and fruits of chick pea and tomato.
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