Factors affecting shoot regeneration in rought lemon (Citrus jambhiri Lush) rootstock
2010
Saini, H.K. | Gill, M.L.S.
The aim of this work was to standardize tissue culture parameters for gene transfer in rough lemon rootstock. Epicotyl segments were cultured with different concentrations of 6-benzylaminopurine (HAP), cefotaxime and hygromycin. Epicotyl segments (1-2 cm) from etiolated seedlings transferred to 16 h photoperiod for 2 weeks produced maximum number of adventitious shoot buds on MS ,medium supplemented with BAP (0.5 mgl-I). Longitudinal cut gave the highest number of buds per explant as compared to transverse cut. Maximum bud induction frequency was obtained on MS + BAP (0.5 mgl-I) medium. The sensitivity of epicotyl segments cultured on regeneration medium MS + BAP (0.5 mgl-I) to antibiotics, cefotaxime and hygromycin was observed at concentration of 400 ppm and 2 mgl-I, respectively. The study can help in developing transgenics of rough lemon rootstock.
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