Detection of Sarcocystis spp. of slaughtered sheep in Gazvin Ziaran slaughter house by molecular assay.
2008
Dalimi Asl, A`bd Ol-Hossein | Paykari, Habib Ol-Lah | Vali Zadeh, Mohsen | Karimi, Gholam Reza | Mutamedi, Gholam Reza | A`bdi Gudarzi, Mohammad | Esmaeil Zadeh, Majid | Meshkat, Mohsen | Najjar, A`sam
Introduction Sarcocystosis is caused by species of Sarcocystis, an intracellular protozoan parasite in the phylum Apicomplexa. The intermediate hosts are the herbivores eating infected food or water containing sporocysts excreted by the carnivorous final host, and that result in tissue cysts in corpses. Method and Material For the present study 60 specimens from the diaphragm, heart and esophagus muscles of sheep slaughtered in Qazvin Ziaran slaughter house were collected. 40 specimens contained macroscopic Sarcocystis cysts and the next 20 contained microscopic Sarcocystis cysts. They were examined by Dob Smear and digestion method. DNA extraction was carried out by kit. PCR conditions were prepared for the 18S rRNA amplification. Meanwhile, a comparative study was done to investigate the specific primers of the Toxoplasma and Neospora along with the Sarcocystis cases. According to the position of restriction, restricted enzymes were selected. Results Results indicated that the primers were completely unique and specific for detecting Sarcocystis. PCR-RFLP analysis in the cases with EcoRI, AvaI, HincII, and TaqI enzymes showed that macroscopic cysts and microscopic cysts belonged to Sarcocystis gigantea and Sarcocystis areticanis, respectively.
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