Isolation and Identification of the Infectious Haematopoietic Necrosis Virus (IHNV)-like Agent from Farmed Rainbow Trout (Oncorhynchus mykiss) from Iran
2003
Fallahi, R., Razi Vaccine and Serum Research Institute;Tehran, Iran | Soltani, M., Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | Kargar, R., Razi Vaccine and Serum Research Institute; Tehran, Iran | Zorriehzahra, M.E.J., Fish Health Unit, Iranian Fisheries Research Institute, Tehran, Iran | Shchelkunov, I., All-Russian Research Institute of Freshwater Fisheries, Rybnoye, Dmitrov Region, Moscow, Russia | Hemmatzadeh, F, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | Nouri, A., Razi Vaccine and Serum Research Institute; Tehran, Iran
The isolation of viral causative agent of fry trout mortality syndrome in farmed rainbow trout in Iran was discussed. Four hundred twenty samples were collected from six provinces for virological, serological and electron microscopic examinations from October 2001 till May 2002. No cytopathic effect (CPE) was observed in the cell cultures except for EPC and BF-2 cells in which signs of CPE appeared one day postinoculation during the second and third passages of two brooder ovarian fluids. Electron microscopic examination revealed the viral particles measured 67-73×128-182nm in the pelleted materials from the CPE positive in BF-2 and EPC cell lines. Indirect immunoflurecence antibody test were positive for some of smears obtained from kidney tissues of the larvae, the samples of ovarian fluids that showed CPE on EPC and BF-2 cell lines as well as CPE positive cell cultures. Using ELISA eight of all sera samples tested were positive with optical density ranged 0.316-2.268 compared to 0.06 for negative controls.
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