PREPARATION OF MONOCLONAL ANTIBODY TO FUSION GLYCOPROTEIN OF NEWCASTLE DISEASE VIRUS ISOLATED FROM IRAN
2002
Madani, R., Razi Vaccine and Serum Research Institute, Tehran, Iran | Golchinfar,F., Razi Vaccine and Serum Research Institute, Tehran, Iran | Purbakhsh,S A., Avian Diseases Research and Diagnosis Dept,. Razi Institue | Frozandeh Moghadam, M., Medeical school Tarbiat Modarres Univercity. Tehran. Iran
Ten Hybridomas secreting monoclonal antibodies directed against the F-glycoprotein of MK13 (Iranian Isolate) strain of Newcastle disease virus (NDV) has isolated which 6 of them showed positive reaction in different tests. All of these antibodies are IgM class with No.k, chain which showed sharp and single band on F-Protein (56000 Dalton) in western blot assay. All these antibodies reacted with same epitope on competitive ELISA. On cross reactivity analysis with different avian viruses all were specific to NDV only and did not show any reaction with other virus. the importance and application of these antibodies would be on specific identification of NDV, highly purification of F-protein of NDV specially on preparation of recombinant F-protein on subunit vaccine, developing anti-idiotype vaccine and also blocking the cleavage site of F-protein which resulting in decreasing pathogenicity of virus.
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