Preparation Elisa Kit For The M easurment Of Epsilon And Beta Antitoxin In The Serum Of Vaccinated Sheep With Enterotoxaemia Vaccine
1999
Farzan , Abdol Vahhab
The major types of clostridium perfringens D,B and C are the aetiologents of enterotoxaemia (pulpy Kidney) of sheep, lambdysentry, haemorrhagic enteritis and struck in sheep. Enterotoxaemia is controlled by million doses of polyvalent vaccine which is produced by Iranian researcher in Razi Institute. After vaccination, there is enough amount of epsilon and beta antitoxin present in sheep sera that prevented the animals against the disease. The antitoxins are measured in vivo using serum neutralization test (SN) in mice, that is a prolonged method. An Enzymelinked Immunosorbent Assay (ELIZA) has been developed as an alternative to SN in mice to measure epsilon and beta antitoxin. by this method, seroepidemiological studies are practicaL In this research we have tried to prapare and to standardize the major components of specific ELlSA Kit, including Epsilon prototoxin, Beta toxin and conjugated Rabbit Anti sheep IgG. Then we measured Epsilon and Beta antitoxin in seven sheep sera by ELISA and SN. For ELISA titration, the standard curves were built using the standard epsilon and beta antitoxin provided by weybridge, U.K and COPENHAGEN, DENMARK The titration results by ELISA and SN were compared using the correlation coefficients.The best correlation between SN and ELISA was found using of 0.2 mg/ml epsilonprototoxin and beta toxin. The assays by ELISA were found to be rapid, specific and economical. ELISA is a useful method to evaluate enterotoxaemia vaccines in the field. In this way, we can titrate large number of sera in a short period of time.
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