Culture medium for pig pre-implantation embryos
2009
Sasaki, Y., Tokyo Univ. of Agriculture, Atsugi, Kanagawa (Japan) | Iwata, H. | Monji, Y. | Kuwayama, T.
The aim of the present study was to create a new culture medium for post-hatched porcine pre-implantation embryos. Porcine embryos were collected from superovulated sows 12 days after insemination. Uteri were surgically removed from the superovulated sows and the uterine fluid (UF) were collected for measurement of the mineral, amino acid and energetic substrate composition. We then created a basal medium with a mineral and energy substrate composition resembling that of the UF and supplemented it with essential and non-essential amino acids. Using this basal medium, we examined the effect of supplementation with fetal bovine serum (FBS), bovine serum albumin (BSA), or both on embryo development. Embryo development, determined by embryo diameter was mostly maintained in the medium containing 1% FBS and 5 mg/ml BSA. The effect of medium supplementation with various concentrations (0, 5, 10 mM) of amino acids found at high concentrations in the UF (serine, glycine, taurine and glutamic acid) on embryo development was examined. Supplementation of the medium with either 5 mM or 10 mM of each amino acid improved embryo development. The addition of citric acid to the medium had no effect on embryo development. These finding suggest that supplementation of the basal medium with 1% FBS 5 mg/ml BSA, and 5 mM of each amino acid (serine, glycine, taurine and glutamic acid) is an optimal culture condition for day-12 porcine post-hatched preimplantation embryos.
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