A new method for the quantification of HMF in honey
2010
Yada, H., National Food Research Inst., Tsukuba, Ibaraki (Japan) | Yanaka, K. | Ohnishi Kameyama, M.
The method of HPLC sample preparation for 5-hydorxymethyl-2-furfural (HMF) quantification in honey was developed. This method carried out as follows, dilute honey with ethanol/water =70/30 (v/v), centrifuge the solution, and filtrate the centrifugal supernatant with 0.2 microm membrane filter. HPLC samples prepared from the filtrate by dilution with water, which were indicated stable analytical values of HMF during one-night (16hrs). This method is useful for routine HMF quantification in honey, because no waste solution containing zinc acetate. We also described the HPLC condition, gradient elution with C18 column/ detection at 254 nm and 284 nm. This HPLC condition make easy to detect the peak which changed from HMF.
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