A new method for measuring gas exchange rates of plant tissue culture vessels
2010
Ito, R., Gifu Univ. (Japan) | Shimazu, T. | Tanaka, I.
A new method for measuring the gas exchange rates of plant tissue culture vessels was developed using the same assumption used in previous models; namely, the air inside the vessel is completely mixed. In this new method, the gas exchange rate was evaluated from the mole fraction of water vapor inside and outside the vessels and the evaporation rate of water inside the vessel measured by economical measuring devices. To examine the mixing state of the air inside the vessel, humidity distributions in the vertical direction of two vessels of different diameters were measured. The humidity distribution in the 10 cm diameter vessel was uniform; however, the humidity distribution in the 2.5 cm diameter vessel was not. This result suggested that the assumption of complete mixing of air was valid only for larger diameter vessels; hence methods to measure gas exchange based on this assumption are limited to larger diameter vessels. Subsequently, the gas exchange rates of vessels 10 cm in diameter were measured using our new method and three existing.methods. The results suggest that the values obtained by this method agree well with those obtained by measuring the change in CO2 gas concentrations inside vessels as a function of time using gas chromatography, if the gas exchange results from diffusion caused by a concentration difference between the inside and outside of the vessel.
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