Simultaneous Determination of Quinolones in Flatfish and Egg Using liquid Chromatography with Fluorescence Detection
2008
Lee, S.H. (Food Analysis Center, Korea Food Research Institute, Seongnam, Republic of Korea) | Shim, Y.S. (Food Analysis Center, Korea Food Research Institute, Seongnam, Republic of Korea) | Kim, H.J. (Food Analysis Center, Korea Food Research Institute, Seongnam, Republic of Korea) | Choi, Y.H. (Food Analysis Center, Korea Food Research Institute, Seongnam, Republic of Korea) | Shin, D.B. (Food Analysis Center, Korea Food Research Institute, Seongnam, Republic of Korea), E-mail: [email protected]
An analytical method for the simultaneous determination of nine quinolones (QNs) namely, marbofloxacin, norfloxacin(IS), ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, and flumequine in flatfish and egg was developed and validated using liquid chromatography with fluorescence detection (LC-FD). The samples were extracted using a traditional liquid-liquid extraction process; deproteinization was accomplished by the addition of trichloroacetic acid and acetonitrile (ACN), and defatting was performed with hexane. Chromatographic separation was achieved on a reverse phase C8 column with gradient elution using a mobile phase of 200 mM ammonium acetate buffer (pH 4.5) and ACN. The proposed method was validated according to the CODEX guideline. Mean recoveries of QNs from flatfish and egg were 89.6-106.5% with relative standard deviations (RSDs) below 15% at three different concentrations of 50, 100 and 500 ㎍/kg. Linearity was obtained with a correlation coefficient (r²) of 0.9989-1.0000. The LOD for the investigated QNs was 1-16 ㎍/kg depending on flatfish and egg. The present method can be applied simultaneously to determine QNs in muscle of flatfish and egg.
显示更多 [+] 显示较少 [-]