Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schlegeli in Korea
2008
Joh, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: johsj@nvrqs.go.kr | Lee, Y.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, C.S. (Konkuk University, Seoul, Republic of Korea) | Kang, S.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Mo, I.P. (Chungbuk National University, Cheongju, Republic of Korea) | Heo, G.J. (Chungbuk National University, Cheongju, Republic of Korea)
The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.
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