Development of a Nutrient-circulated Micropropagation System and Growth of Potato Plantlets under Different Numbers of Air Exchanges
2009
Son, J.E. (Seoul National University, Seoul, Republic of Korea), E-mail: [email protected] | Jang, I. (Seoul National University, Seoul, Republic of Korea) | Park, H.J. (Seoul National University, Seoul, Republic of Korea) | Oh, M.M. (Kansas State University, Manhattan, USA)
A nutrient-circulated micropropagation system using a scaled-up vessel was developed for photoautotrophic micropropagation of potato plantlets. To verify usefulness of this system, potato (Solanum tuberosum L. cv. Dejima) plantlets were micropropagated under three different systems: nutrient-circulated photoautotrophic micropropagation system using a scaled-up vessel (PAS), photoautotrophic micropropagation using a magenta vessel (PAM), and photomixotrophic micropropagation using a magenta vessel (PMM). The growth of potato plantlets under PAS was significantly improved compared to those under PAM and PMM due to adequate CO₂ supply by forced ventilation and better root-zone environment conditions through circulated nutrient solution. This result on in vitro growth directly affected to survival rate and ex vitro growth. In addition, to determine proper ventilation rate in PAS, potato plantlets were grown under scaled-up vessels with different numbers of air exchanges (4, 7, 10, and 13 h-¹). The number of air exchanges had an impact on relative humidity rather than CO₂ concentration in the vessels. Low relative humidity (70-80%) by high numbers of air exchanges (10, and 13 h-¹) induced well-developed guard cells and wax deposition on the leaves of potato plantlets. Furthermore, high numbers of air exchanges (10, and 13 h-¹) promoted in vitro growth and subsequently ex vitro growth after transplanting. Thus, it is concluded that PAS was suitable for the mass production of potato plantlets; also, to obtain vigorous growth of potato plantlets cultured in PAS, the number of air exchanges should be set at 10 h-¹ or over by forced ventilation.
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