Molecular cloning, tissue distribution and quantitative analysis of two proopiomelanocortin mRNAs in Japanese flounder (Paralichthys olivaceus)
2009
Kim, K.S. (Pukyong National University, Busan, Republic of Korea) | Kim, H.W. (Pukyong National University, Busan, Republic of Korea) | Chen, Thomas T. (University of Connecticut, Storrs, CT, USA) | Kim, Y.T. (Pukyong National University, Busan, Republic of Korea), E-mail: [email protected]
Proopiomelanocortin (POMC) plays an essential role in the stress response of the hypothalamic-pituitary-adrenal axis, and is the precursor of biologically active peptides such as adrenocorticotropin (ACTH), α-melanocyte-stimulating hormone (α-MSH), β-melanocyte-stimulation hormone (β-MSH) and β-endorphin. We have synthesized two different forms of POMC cDNA clones, POMC-Ⅰ and POMC-Ⅱ, from a pituitary cDNA library for Paralichthys olivaceus, or Japanese flounder. jfPOMC-Ⅰ cDNA consists of 954bp and encodes a polypeptide of 216 amino acid residues, whereas jfPOMC-Ⅱ consists of 971bp which encode a polypeptide of 194 amino acid residues. The high levels of jfPOMC-Ⅰ and -Ⅱ mRNAs detected in the pituitary tissue and moderate levels detected in the brain tissue plus our quantitative RT-PCR analysis, which showed there to be no significant difference between the levels of jfPOMC-Ⅰ and -Ⅱ mRNAs, indicate that there may be no functional separation between these two mRNAs in the flounder.
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