Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium
2009
Goswami, Debajyoti (Indian Institute of Technology Kharagpur, Kharagpur, India) | Basu, Kumar Jayanta (Indian Institute of Technology Kharagpur, Kharagpur, India) | De, Sirshendu (Indian Institute of Technology Kharagpur, Kharagpur, India), E-mail: [email protected]
In this study, castor oil is hydrolyzed in presence of Candida rugosa lipase, while in the buffer (aqueous) phase as a dispersion medium. The following conditions were used to optimize the process: speed of agitation, initial pH of buffer phase, temperature, and ratio of buffer phase volume to oil weight. The optimal conditions are 1,100 rpm, pH 6.5, temperature 35℃, and 3:1 buffer phase volume to oil weight ratio. Under these described conditions, the reusability of lipase was tested and it was found that nearly 80% of original hydrolysis percentage was achieved after the first recycle.
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