Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung [Ipomoea aquatica] hairy roots as a sensing tool
2010
Nishioka, M., Osaka Univ., Toyonaka (Japan) | Ojima, Y. | Tsushima, Y. | Matsumoto, M. | Taya, M.
In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/square m) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, Rsub(D), can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the Rsub(D), while lowering the content of ROS and moderating the DNA injury.
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