Antioxidative and Antimicrobial Activities of Eriobotrya japonica Lindl. Leaf Extracts
2009
Lee, K.I., Dongshin University, Naju, Republic of Korea | Kim, S.M., Dongshin University, Naju, Republic of Korea
Antioxidative, antimicrobial activities and Raw 264.7 cell viability as cytotoxicity of various solvent extracts from leaf of Eriobotrya japonica Lindl. dried by different methods were investigated for processing as functional ingredient. In DPPH radical scavenging activity, RLE (80% EtOH extract of raw leaf) and FLE (80% EtOH extract of freeze-dried leaf) exhibited strong scavenging effect on 300 μM DPPH radical solution (1.71 mg/mL and 2.11 mg/mL for RLE SC∧50 and FLE SC∧50). Also in nitric oxide scavenging activity, RLE and FLE showed strong activities (83.9% and 82.2% in 5 mg/mL sample concentration). Total phenolic compound contents of each extracts were found to be 73.7~215.4 mg/g and RLE was showed the highest phenolic compound content. Also, total flavonoid contents were found to be 24.85~110.3 mg/g and RLE was showed the highest flavonoid content. In antimicrobial activity, RLE was showed higher growth inhibition effect against all microbial strains. RLE, RLW (hot water extract of raw leaf), and FLW (hot water extract of freeze-dried leaf) exhibited strong antimicrobial activities against MRSA and S. aureus. In measurement of cytotoxicity by MTT assay, Raw 264.7 cell viabilities of 80% EtOH extracts showed better effect than water extracts. Especially viability of RLE was found be over 100% in every tested sample concentration.
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